hy 19991  (MedChemExpress)

Journal: Current Issues in Molecular Biology

Article Title: Walnut Green Husk Polysaccharide Improve Gut Microbiota and Alleviate Intestinal Inflammation Caused by Immune Checkpoint Inhibitors

doi: 10.3390/cimb48020195

Figure Lengend Snippet: Walnut green husk polysaccharides (WGHP) regulates the composition of intestinal microbiota destroyed by PD-1 inhibitors. ( A ): Venn diagram of gut microbiota in each group of mice; ( B ): PCOA (Principal Coordinates Analysis); ( C ): LEfSe (Linear discriminant analysis Effect Size) Differential Species Abundance Bar Chart; ( D ): LefSe (LEfSe (LDA Score Plot); ( E ): Histogram of the phylum; ( F ): Relative abundance of Firmicutes; ( G ): Relative abundance of Bacteroideta; ( H ): The ratio of Firmicutes/Bacteroideta. Compared with NC, * p < 0.05, ** p < 0.01, *** p < 0.001, ns stands for p > 0.05, compared with MC, # p < 0.05, ## p < 0.01, ### p < 0.001.

Article Snippet: The following reagents were used: SCFAs (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), enzyme-linked immunosorbent assay (ELISA) kits for tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-22 (IL-22) (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China), PD-1 inhibitors (MCE, Monmouth Junction, NJ, USA, HY-19991), corn oil (Biyuntian Biotechnology Co., Ltd., Shanghai, China), bicinchoninic acid (BCA) protein concentration assay kit (Shanghai Biyuntian Biotechnology Co., Ltd., Shanghai, China), enhanced RIPA tissue lysate, anti-GAPDH antibody (BM3896), HRP-conjugated goat anti-rabbit IgG, anti-PD-L1/CD274 antibody, and anti-GPR43 antibody (Beijing Braitex Biotechnology Co., Ltd., Beijing, China), and protease phosphatase inhibitor cocktail (general-purpose, 50X) (Biyuntian Biotechnology Co., Ltd., Shanghai, China).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Walnut Green Husk Polysaccharide Improve Gut Microbiota and Alleviate Intestinal Inflammation Caused by Immune Checkpoint Inhibitors

doi: 10.3390/cimb48020195

Figure Lengend Snippet: Concentration of SCFAs (short chain fatty acids) in the Mouse Intestine and Expression of related proteins in colon. ( A ): Acetic acid, ( B ): Propionic Acid, ( C ): Butyric acid, ( D ): Isobutyric acid, ( E ): Valeric acid, ( F ): Isovaleric acid, ( G – J ): Protein quantification of PD-1, PD-L1, and GPR43, respectively. Compared with NC, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for p > 0.05, compared with MC, # p < 0.05, ## p < 0.01, ### p < 0.001.

Article Snippet: The following reagents were used: SCFAs (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), enzyme-linked immunosorbent assay (ELISA) kits for tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-22 (IL-22) (Shanghai Enzyme-linked Biotechnology Co., Ltd., Shanghai, China), PD-1 inhibitors (MCE, Monmouth Junction, NJ, USA, HY-19991), corn oil (Biyuntian Biotechnology Co., Ltd., Shanghai, China), bicinchoninic acid (BCA) protein concentration assay kit (Shanghai Biyuntian Biotechnology Co., Ltd., Shanghai, China), enhanced RIPA tissue lysate, anti-GAPDH antibody (BM3896), HRP-conjugated goat anti-rabbit IgG, anti-PD-L1/CD274 antibody, and anti-GPR43 antibody (Beijing Braitex Biotechnology Co., Ltd., Beijing, China), and protease phosphatase inhibitor cocktail (general-purpose, 50X) (Biyuntian Biotechnology Co., Ltd., Shanghai, China).

Techniques: Concentration Assay, Expressing

Journal: Journal of Advanced Research

Article Title: An injectable nano-hydroxyapatite-incorporated hydrogel with sustained release of Notoginsenoside R1 enhances bone regeneration by promoting angiogenesis through Notch1/Akt signaling

doi: 10.1016/j.jare.2025.05.025

Figure Lengend Snippet: (A) The angiogenic-related gene expression quantified by qRT-PCR at day 4, day 7 and day 10. (B) The angiogenic proteins of VEGF and VEGFR-2 analyzed by western blot. (C)semiquantitative analysis of VEGF and VEGFR-2 protein expression. (D) The critical markers Notch1, Hes1 involved in the Notch 1 pathway analyzed by western blot and their semiquantitative analysis (E).(F)Western blot and semiquantitative analysis (G) of Akt protein expression.(H)The gene expression of Akt and VEGF under NGR1 stimulation with Notch 1 inhibition. (I) The gene expression of Notch 1 and VEGF under NGR1 stimulation with Akt inhibition. (J) Western blot and semiquantitative analysis (K) of Akt protein expression induced by NGR1 under Notch 1 inhibition. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant.

Article Snippet: Subsequently, Notch 1 inhibitor (HY-1302, MCE, USA) and Akt inhibitor (HY-10358, MCE, USA) were applied to evaluate their effects on angiogenic-related gene expression.

Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Expressing, Inhibition